The first records of Bolevec date back to 1382. However, the village was probably founded in the 12^th century, when many monasteries were established with the task to colonize large areas. The village belonged to the Church of St. Apollinaris in Prague's New Town. In 1460 it was sold to the city of Pilsen for 200 schocks of Prague groschen. This sale was approved directly by the Czech king George of Podebrady. The city of Pilsen bought Bolevec with the intention of building a large pond system there. Due to the flooding of the farmers' land, the village was granted various privileges. In 1461, the village contained 10 homesteads and one of them stood on the site of today's farmstead “U Matoušů”, but at that time it was called Weysovsky farmstead. The owner was in charge between 1635–1684 Matouš Vicl, after whom the farmstead is called “U Matoušů”.

During the 19^th century, the entire farmstead was significantly rebuilt. In 1809 the present residential house was constructed, with part of the walls using the original wooden structure of the older house. Stables were built with greater capacity and in addition, the barn was also extended. The arched gates bear the dates 1830 and 1834. A new granary was also built in the 1840s to replace the older smaller granary. The last significant alteration was the addition of a shed to the barn in 1925.

In 1952 a collective farm was established in Bolevec,  where the collective farm took over the farm buildings themselves. In the stables, they set up a pig-house. The last owners were allowed to stay in the house to oversee the pigeons and poultry. In 1976 the collective farm was closed down due to the construction of a housing estate. Originally, the entire Bolevec village square was supposed to be completely removed to this project, and only the farmstead "U Matoušů" as a registered monument should have remained preserved. Fortunately, through the hard work of several activists, the Bolevec village square remained preserved.

In the 1980s, the conversion of the entire area to a Young Breeders and Conservationists Station was considered. The state even bought the site for this purpose at a very low price. However, the initiated works were stopped by the events of 1989. After the Velvet Revolution, the entire farmstead was returned to the original owners due to the unfavourable and forced sale. At that time, the farmstead was already in poor condition and the thatched roofing had practically disappeared. In 2004, the town of Plzen managed to purchase the site and in 2007 it was transferred to the state under the administration of the National Heritage Institute. In 2017, the project "Year in the Village" was approved, under which the structural restoration and revitalization of the entire farmstead began in August 2019 and was fully completed in early 2021.

On 1^st June 2021 farmstead "U Matoušů" welcomed its first visitors.